Functions as histone acetyltransferase and regulates transcription via chromatin remodeling.
Acetylates all four core histones in nucleosomes.
Histone acetylation gives an epigenetic tag for transcriptional activation.
Mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein.
Mediates acetylation of histone H3 at 'Lys-122' (H3K122ac), a modification that localizes at the surface of the histone octamer and stimulates transcription, possibly by promoting nucleosome instability.
Mediates acetylation of histone H3 at 'Lys-27' (H3K27ac).
Also functions as acetyltransferase for nonhistone targets.
Acetylates 'Lys-131' of ALX1 and acts as its coactivator in the presence of CREBBP.
Acetylates SIRT2 and is proposed to indirectly increase the transcriptional activity of TP53 through acetylation and subsequent attenuation of SIRT2 deacetylase function.
Acetylates HDAC1 leading to its inactivation and modulation of transcription.
Acts as a TFAP2A-mediated transcriptional coactivator in presence of CITED2.
Plays a role as a coactivator of NEUROD1-dependent transcription of the secretin and p21 genes and controls terminal differentiation of cells in the intestinal epithelium.
Promotes cardiac myocyte enlargement.
Can also mediate transcriptional repression.
Binds to and may be involved in the transforming capacity of the adenovirus E1A protein.
In case of HIV-1 infection, it is recruited by the viral protein Tat.
Regulates Tat's transactivating activity and may help inducing chromatin remodeling of proviral genes.
Acetylates FOXO1 and enhances its transcriptional activity.
Acetylates BCL6 wich disrupts its ability to recruit histone deacetylases and hinders its transcriptional repressor activity.
Participates in CLOCK or NPAS2-regulated rhythmic gene transcription; exhibits a circadian association with CLOCK or NPAS2, correlating with increase in PER1/2 mRNA and histone H3 acetylation on the PER1/2 promoter.
Acetylates MTA1 at 'Lys-626' which is essential for its transcriptional coactivator activity.
Acetylates XBP1 isoform 2; acetylation increases protein stability of XBP1 isoform 2 and enhances its transcriptional activity (By similarity).
Applications:Suitable for use in ELISA, Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:2000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity：Recognizes human EP300.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：KLH-conjugated synthetic peptide mapping to a fragment of residues within aa1807-1841 of human EP300.
