Non-receptor tyrosine kinase indispensable for B lymphocyte development, differentiation and signaling.
Binding of antigen to the B-cell antigen receptor (BCR) triggers signaling that ultimately leads to B-cell activation.
After BCR engagement and activation at the plasma membrane, phosphorylates PLCG2 at several sites, igniting the downstream signaling pathway through calcium mobilization, followed by activation of the protein kinase C (PKC) family members.
PLCG2 phosphorylation is performed in close cooperation with the adapter protein B-cell linker protein BLNK.
BTK acts as a platform to bring together a diverse array of signaling proteins and is implicated in cytokine receptor signaling pathways.
Plays an important role in the function of immune cells of innate as well as adaptive immunity, as a component of the Toll-like receptors (TLR) pathway.
The TLR pathway acts as a primary surveillance system for the detection of pathogens and are crucial to the activation of host defense.
Especially, is a critical molecule in regulating TLR9 activation in splenic B-cells.
Within the TLR pathway, induces tyrosine phosphorylation of TIRAP which leads to TIRAP degradation.
BTK plays also a critical role in transcription regulation.
Induces the activity of NF-kappa-B, which is involved in regulating the expression of hundreds of genes.
BTK is involved on the signaling pathway linking TLR8 and TLR9 to NF-kappa-B.
Transiently phosphorylates transcription factor GTF2I on tyrosine residues in response to BCR.
GTF2I then translocates to the nucleus to bind regulatory enhancer elements to modulate gene expression.
ARID3A and NFAT are other transcriptional target of BTK.
BTK is required for the formation of functional ARID3A DNA-binding complexes.
There is however no evidence that BTK itself binds directly to DNA.
BTK has a dual role in the regulation of apoptosis.
Applications:Suitable for use in ELISA, Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity：Recognizes human BTK. Species Crossreactivity: mouse.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo
Immunogen：KLH-conjugated synthetic peptide mapping to a fragment of residues within aa396-430 of the Central region of human BTK. Species sequence homology: chicken.
