DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs.
Pol II is the central component of the basal RNA polymerase II transcription machinery.
It is composed of mobile elements that move relative to each other.
RPB3 is part of the core element with the central large cleft and the clamp element that moves to open and close the cleft (By similarity).
Applications:Suitable for use in Immunofluorescence, ELISA, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:25Western Blot: 1:1000Immunohistochemistry (Paraffin): 1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:200ul
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by Protein A and peptide affinity chromatography.
Form:Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:Recognizes human POLR2C. Species Crossreactivity: mouse.
Isotype:IgG
Calc Applications Abbrev:E IF IHC WB
Calc Crossreactivity:Hu Mo
Immunogen:KLH-conjugated synthetic peptide mapping to a fragment of residues within aa200-228 of the C-terminal region of human POLR2C. Species sequence homology: bovine.