An outbreak of atypical pneumonia, referred to as severe acute respiratory syndrome (SARS) and first identified in Guangdong Province, China, has spread to several countries.
The severity of this disease is such that the mortality rate appears to be ~3 to 6%.
A number of laboratories worldwidehave undertaken the identification of the causative agent.
The National Microbiology Laboratory in Canada obtained the Tor2 isolate from a patient in Toronto, and succeeded in growing a coronavirus-like agent in African Green Monkey Kidney (Vero E6) cells.
This coronavirus has been named publicly by the World Health Organization and member laboratories as SARS virus.
The SARS membrane proteins, including the major proteins S (Spike) and M (Membrane), are inserted into the endoplasmic reticulum Golgi intermediate compartment (ERGIC) while full length replicated RNA (+ strands) assemble with the N (nucleocapsid) protein.
The virus then migrates through the Golgi complex and eventually exits the cell, likely by exocytosis.
The site of viral attachment to the host cell resides within the S protein.
Oligomeric spike (S) glycoproteins extend from SARS membranes.
These integral membrane proteins assemble within the endoplasmic reticulum of infected cells and are subsequently endoproteolyzed in the Golgi, generating noncovalently associated S1 and S2 fragments.
Once on the surface of infected cells and virions, peripheral S1 fragments bind carcinoembryonic antigen-related cell adhesion molecule (CEACAM) receptors, and this triggers membrane fusion reactions mediated by integral membrane S2 fragments.
Applications:Suitable for use in ELISA.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Grade：Purified
Purity：Purified by ammonium sulfate precipitation.
Form：Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity：Recognizes SARS virus PUPM.
Isotype：IgG
Calc Applications Abbrev：E
Immunogen：KLH-conjugated synthetic peptide mapping to a fragment of residues within aa192-221 of the C-terminal region of SARS M protein.
