The CD133 gene codes for a pentaspan transmembrane glycoprotein.
The CD133 antigen appears to belong to a new molecular family of 5-TM proteins, as the characterization of the CD133 antigen and prominin in the mouse were the first descriptions of a 5-TM glycoprotein structure.
This 'family' includes members from several different species (which may be homologs) including human, mouse, rat, fly, and worm.
The 5-TM structure includes an extracellular N-terminal, two short intracellular loops, two large extracellular loops and an intracellular C-terminal CD133 was initially shown to be expressed on primitive hematopoietic stem and progenitor cells and retinoblastoma.
CD133 has since been shown to be expressed on hemangioblasts, and neural stem cells as well as on developing epithelium.
Expression patterns for CD133 generally mimic those of the murine prominin molecule, although CD133 antigen has not yet been demonstrated on adult epithelial tissue.
The CD133 positive fraction of human bone marrow, cord blood and peripheral blood have been shown to efficiently engraft in xenotransplantation models, and have been shown to contain the majority of the granulocyte/macrophage precursors, NOD/SCID repopulating cells and CD34 + dendritic cell precursors.
Phenotypically, CD133 positive cells in blood and marrow are CD34 bright, with CD34 dim CD71 bright cells being negative for CD133 expression.
Many leukemias express CD133 as well as CD34 , but some investigators have noted leukemic blasts which are CD133+ and CD34 negative.
No natural ligand has yet been demonstrated for the CD133 molecule, and its function in hematopoietic tissue is unknown.
Applications:Suitable for use in Immunofluorescence, Indirect Flow Cytometry, ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:10-1:50Indirect Flow Cytometry: 1:10-1:50Western Blot: 1:1000Immunohistochemistry (Paraffin): 1:50-1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified by ammonium sulfate precipitation.
Form：Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity：Recognizes human AC133.
Isotype：IgG
Calc Applications Abbrev：E FC IF IHC WB
Calc Crossreactivity：Hu
Immunogen：KLH-conjugated, synthetic peptide corresponding to the C-terminal region of human AC133.
