UPF3B is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance.
The protein is one of two functional homologs to yeast Upf3p.
This protein binds to the mRNA and remains bound after nuclear export, acting as a nucleocytoplasmic shuttling protein.
It forms with Y14 a complex that binds specifically 20 nt upstream of exon-exon junctions This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance.
The encoded protein is one of two functional homologs to yeast Upf3p.
mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD).
When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons.
This protein binds to the mRNA and remains bound after nuclear export, acting as a nucleocytoplasmic shuttling protein.
It forms with Y14 a complex that binds specifically 20 nt upstream of exon-exon junctions.
This gene is located on the long arm of chromosome X.
Two splice variants encoding different isoforms have been found for this gene.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, 2% sucrose, 0.09% sodium azide.
Specificity：Recognizes human UPF3B.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to the middle region of human UPF3B
