Control of transcription by RNA polymerase II involves the basal transcription machinery which is a collection of proteins.
These proteins with RNA polymerase II, assemble into complexes which are modulated by transactivator proteins that bind to cis-regulatory elements located adjacent to the transcription start site.
Some modulators interact directly with the basal complex, whereas others may act as bridging proteins linking transactivators to the basal transcription factors.
Some of these associated factors are weakly attached while others are tightly associated with TBP in the TFIID complex.
Among the latter are the TAF proteins.
Different TAFs are predicted to mediate the function of distinct transcriptional activators for a variety of gene promoters and RNA polymerases.
TAF12 interacts directly with TBP as well as with TAF2I.
Control of transcription by RNA polymerase II involves the basal transcription machinery which is a collection of proteins.
These proteins with RNA polymerase II, assemble into complexes which are modulated by transactivator proteins that bind to cis-regulatory elements located adjacent to the transcription start site.
Some modulators interact directly with the basal complex, whereas others may act as bridging proteins linking transactivators to the basal transcription factors.
Some of these associated factors are weakly attached while others are tightly associated with TBP in the TFIID complex.
Among the latter are the TAF proteins.
Different TAFs are predicted to mediate the function of distinct transcriptional activators for a variety of gene promoters and RNA polymerases.
TAF12 interacts directly with TBP as well as with TAF2I.
Applications:Suitable for use in Western Blot, CHIP.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, 2% sucrose, 0.09% sodium azide.
Specificity：Recognizes human TAF12.
Isotype：IgG
Calc Applications Abbrev：ChIP WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to the N-terminal region of human TAF12
