84-5037-05　PRKAR1A (cAMP-Dependent Protein Kinase Type L-alpha Regulatory Subunit, Protein Kinase, cAMP-Dependent, Regulatory, Type I, Alpha (Tissue Specific Extinguisher 1)) 100ul　489370

特徴

• cAMP is a signaling molecule important for a variety of cellular functions.
• cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins.
• The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits.
• cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits.
• Four different regulatory subunits and three catalytic subunits have been identified in humans.
• This gene encodes one of the regulatory subunits.
• This protein was found to be a tissue-specific extinguisher that down-regulates the expression of seven liver genes in hepatoma x fibroblast hybrids.
• Mutations in this gene cause Carney complex (CNC).
• This gene can fuse to the RET protooncogene by gene rearrangement and form the thyroid tumor-specific chimeric oncogene known as PTC2.
• A nonconventional nuclear localization sequence (NLS) has been found for this protein which suggests a role in DNA replication via the protein serving as a nuclear transport protein for the second subunit of the Replication Factor C (RFC40).
• Three alternatively spliced transcript variants encoding the same protein have been observed.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.