84-5008-49　NARF (Nuclear Prelamin A Recognition Factor) 100ul　486542

特徴

• Several proteins have been found to be prenylated and methylated at their carboxyl-terminal ends.
• Prenylation was initially believed to be important only for membrane attachment.
• However, another role for prenylation appears to be its importance in protein-protein interactions.
• The only nuclear proteins known to be prenylated in mammalian cells are prelamin A- and B-type lamins.
• Prelamin A is farnesylated and carboxymethylated on the cysteine residue of a carboxyl-terminal CaaX motif.
• This post-translationally modified cysteine residue is removed from prelamin A when it is endoproteolytically processed into mature lamin A.
• NARF binds to the prenylated prelamin A carboxyl-terminal tail domain.
• It may be a component of a prelamin A endoprotease complex.
• NARF is located in the nucleus, where it partially colocalizes with the nuclear lamina.
• It shares limited sequence similarity with iron-only bacterial hydrogenases.Several proteins have been found to be prenylated and methylated at their carboxyl-terminal ends.
• Prenylation was initially believed to be important only for membrane attachment.
• However, another role for prenylation appears to be its importance in protein-protein interactions.
• The only nuclear proteins known to be prenylated in mammalian cells are prelamin A- and B-type lamins.
• Prelamin A is farnesylated and carboxymethylated on the cysteine residue of a carboxyl-terminal CaaX motif.
• This post-translationally modified cysteine residue is removed from prelamin A when it is endoproteolytically processed into mature lamin A.
• The protein encoded by this gene binds to the prenylated prelamin A carboxyl-terminal tail domain.
• It may be a component of a prelamin A endoprotease complex.
• The encoded protein is located in the nucleus, where it partially colocalizes with the nuclear lamina.
• It shares limited sequence similarity with iron-only bacterial hydrogenases.
• Alternatively spliced transcript variants encoding different isoforms have been identified for this gene, including one with a novel exon that is generated by RNA editing.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.