JMY acts both as a nuclear p53/TP53-cofactor and a cytoplasmic regulator of actin dynamics depending on conditions.
In nucleus, JMY acts as a cofactor that increases p53/TP53 response via its interaction with p300/EP300.
JMY increases p53/TP53-dependent transcription and apoptosis, suggesting an important role in p53/TP53 stress response such as DNA damage.
In cytoplasm, JMY acts as a nucleation-promoting factor for both branched and unbranched actin filaments.
JMY activates the Arp2/3 complex to induce branched actin filament networks.
JMY also catalyzes actin polymerization in the absence of Arp2/3, creating unbranched filaments.
JMY contributes to cell motility by controlling actin dynamics.
JMY may promote the rapid formation of a branched actin network by first nucleating new mother filaments and then activating Arp2/3 to branch off these filaments.
The p53/TP53-cofactor and actin activator activities are regulated via its subcellular location.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, 2% sucrose, 0.09% sodium azide.
