Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases.
AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites.
Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site.
This gene encodes the major AP endonuclease in human cells.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, 2% sucrose, 0.09% sodium azide.
Specificity：Recognizes APEX1. Species Crossreactivity: bovine, canine, goat, guinea pig, equine, human, mouse, porcine, rabbit, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Bo Ca Eq Gt Gp Hu Mo Po Rb Rt
Immunogen：Synthetic peptide corresponding to the N-terminal region of mouse APEX1
