84-4895-65　AP2M1 100ul　475363

特徴

• AP2M1 is a component of the adaptor protein complex 2 (AP-2).
• Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways.
• It is an adaptor protein which complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation.
• AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin- coated vesicles, CCVs) which are destined for fusion with the early endosome.
• The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components.
• Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation.
• AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis.
• AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface.
• AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.