ADH (alcohol dehydrogenase) family of proteins metabolize a variety of substances such as ethanol, retinal, other aliphatic alcohol, hydroxysteroids, and lipid peroxidation products.
With the coenzyme NAD, ADH catalyzes the reversible conversion of organic alcohol to ketones or aldehydes.
ADH play a major role in ethanol metabolism.
Applications:Suitablee for use in ELISA and Immunoelectrophoresis.
Other applications not tested.
Recommended Dilution:ELISA: 1:20,000-1:100,000Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder is stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O prior to use.
To avoid repeated freezing and thawing, aliquot reconstituted stock solution and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：20mg
Host：rabbit
Grade：Highly Purified
Purity：Purified by Ion Exchange chromatography.
Form：Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% gentamicin sulfate. Reconstitute with sterile dH2O to 2ml. Labeled with horseradish peroxidase (HRP)
Specificity：Recognizes Saccharomyces cerevisiae ALDH. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Alcohol Dehydrogenase (Yeast). Cross reactivity against Alcohol Dehydrogenase from other sources is unknown.
