84-4885-56　PI3 Kinase, p85 (Phosphatidylinositol PI-3 Kinase) 200ul　474213

特徴

• Phosphatidylinositol 3-kinase regulatory subunit alpha (UniProt: Q63787; also known as PI3-kinase regulatory subunit alpha, PI3K regulatory subunit alpha, PtdIns-3-kinase regulatory subunit alpha, Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha, PI3-kinase subunit p85-alpha, PtdIns-3-kinase regulatory subunit p85-alpha) is encoded by the Pik3r1 gene (Gene ID: 25513) in rat.
• PI3K regulatory subunit alpha catalyzes the phosphorylation of PI(4,5)P2 inositol ring at the 3 position to generate PI(3,4,5)P3, a potent second messenger that mediates survival signaling and insulin action.
• PI3 Kinase is a heterodimeric complex composed of an 85 kDa regulatory subunit and a 110kD catalytic subunit.
• Following ligand binding to receptor and activation of receptor tyrosine kinase, the p85/p110 complex is recruited to the receptor by interaction of the SH2 domain of p85 with consensus phosphotyrosine residues on receptor tyrosine kinase.
• This allows the p110 catalytic subunit to come in close proximity to its lipid substrates in the cell membrane.
• The interaction of a receptor tyrosine kinase with the p85 subunit relieves the inhibitory effect of p85 on the p110 catalytic subunit.
• Activated PI 3-kinase phosphorylates phosphoinositol (PI) substrates to produce PI(3)P, PI(3,4)P2, and PI(3,4,5)P3 (PIP3).
• These molecules act as second messengers and recruit PI 3-kinase-dependent serine/threonine kinases (PDK1) and Akt from the cytoplasm to the plasma membrane.
• PI 3-kinase activity is tightly regulated in normal cells.
• Over-activation of this pathway can perturb the control mechanisms for cell growth and survival and contribute to metastatic competence and resistance to chemotherapy.
• Abnormal activation of PI 3-kinases is seen in several forms of cancer.
• About 30% of solid tumors contain mutations in the catalytic unit of their PI 3-kinase.
• PI3 kinase plays a critical role in insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.
• Mutations in Pik3r1 gene are known to be associated with insulin resistance.
• Applications:Suitable for use in Western Blot and Immunoprecipitation.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000 detected PI3-Kinase, p85 in 10ug of Raji cell lysate
• Immunoprecipitation: 2.5ug detected PI3-Kinase, p85 in jurkat cell lysate
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.