84-4882-19　LIMK2 50ug　470055

特徴

• There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain.
• LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers.
• Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions.
• LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain.
• The protein encoded by this gene is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity.
• It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton.
• At least three transcript variants encoding different isoforms have been found for this gene.
• [provided by RefSeq]
• Applications:Suitable for use in Immunofluorescence, Western Blot.
• Other applications not tested.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• AA Sequence:MGSYLSVPAYFTSRDLFRCSECQDSLTNWYYEKDGK LYCPKDYWGKFGEFCHGCSLLMTGPFMVAGEFKYHP ECFACMSCKVIIEDGDAYALVQHATLYCGKCHNEVVLA PMFERLSTESVQEQLPYSVTLISMPATTEGRRGFSVS VESACSNYATTVQVKEVNRMHISPNNRNAIHPGDRILE INGTPVRTLRVEEVEDAISQTSQTLQLLIEHDPVSQRLD QLRLEARLAPHMQNAGHPHALSTLDTKENLEGTLRRR SLRRSNSISKSPGPSSPKEPLLFSRDISRSESLRCSSS YSQQIFRPCDLIHGEVLGKGFFGQAIKVTHKATGKVMV MKELIRCDEETQKTFLTEVKVMRSLDHPNVLKFIGVLY KDKKLNLLTEYIEGGTLKDFLRSMDPFPWQQKVRFAK GIASGMAYLHSMCIIHRDLNSHNCLIKLDKTVVVADFGL SRLIVEERKRAPMEKATTKKRTLRKNDRKKRYTVVGN PYWMAPEMLNGKSYDETVDIFSFGIVLCEIIGQVYADP DCLPRTLDFGLNVKLFWEKFVPTDCPPAFFPLAAICCR LEPESRAPPGAAGEGPGCADDEGPVRRQGKVTIKYD PKELRKHLNLEEWILEQLTRLYDCQEEEISELEIDVDEL LDMESDDAWASRVKELLVDCYKPTEAFISGLLDKIRAM QKLSTPQKK
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.