84-4879-67　VPS4A (Vacuolar Protein Sorting-Associated Protein 4A, Protein SKD2, VPS4-1, hVPS4) 50ul　407307

特徴

• Involved in late steps of the endosomal multivesicular bodies (MVB) pathway.
• Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission.
• Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting.
• MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids.
• In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses).
• Involved in cytokinesis: retained at the midbody by ZFYVE19/ANCHR and CHMP4C until abscission checkpoint signaling is terminated at late cytokinesis.
• It is then released following dephosphorylation of CHMP4C, leading to abscission.
• VPS4A/B are required for the exosomal release of SDCBP, CD63 and syndecan.
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000-1:5000Immunohistochemistry: 1:20-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.