Catalyzes the formation of NAD(+) from nicotinamide mononucleotide (NMN) and ATP.
Can also use the deamidated form; nicotinic acid mononucleotide (NaMN) as substrate with the same efficiency.
Can use triazofurin monophosphate (TrMP) as substrate.
Also catalyzes the reverse reaction, i.e.
the pyrophosphorolytic cleavage of NAD(+).
For the pyrophosphorolytic activity, prefers NAD(+) and NaAD as substrates and degrades NADH, nicotinic acid adenine dinucleotide phosphate (NHD) and nicotinamide guanine dinucleotide (NGD) less effectively.
Fails to cleave phosphorylated dinucleotides NADP(+), NADPH and NaADP(+).
Protects against axonal degeneration following mechanical or toxic insults.
Applications:Suitable for use in Immunofluorescence, ELISA, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:50-1:200Immunohistochemistry: 1:20-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human NMNAT1.
Isotype：IgG
Calc Applications Abbrev：E IF IHC
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to aa1-279 of human NMNAT1.
