84-4869-76　NME4 (Nucleoside Diphosphate Kinase, Mitochondrial, NDK, NDP Kinase, Mitochondrial, Nucleoside Diphosphate Kinase D, NDPKD, Nm23-H4, NM23D) 100ul　406816

特徴

• Major role in the synthesis of nucleoside triphosphates other than ATP.
• The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate.
• Through the catalyzed exchange of gamma-phosphate between di- and triphosphonucleosides participates in regulation of intracellular nucleotide homeostasis.
• Binds to anionic phospholipids, predominantly to cardiolipin; the binding inhibits its phosphotransfer activity.
• Acts as mitochondria-specific NDK; its association with cardiolipin-containing mitochondrial inner membrane is coupled to respiration suggesting that ADP locally regenerated in the mitochondrion innermembrane space by its activity is directly taken up via ANT ADP/ATP translocase into the matrix space to stimulate respiratory ATP regeneration.
• Proposed to increase GTP-loading on dynamin-related GTPase OPA1 in mitochondria.
• In vitro can induce liposome cross-linking suggesting that it can cross-link inner and outer membranes to form contact sites, and promotes intermembrane migration of anionic phosphoplipids.
• Promotes the redistribution of cardiolipin between the mitochondrial inner membrane and outer membrane which is implicated in pro-apoptotic signaling.
• Applications:Suitable for use in ELISA, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Immunohistochemistry: 1:20-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.