Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis.
Complex I functions in the transfer of electrons from NADH to the respiratory chain.
The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Applications:Suitable for use in ELISA, Western Blot, Immunofluorescence, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunofluorescence: 1:50-1:200Immunohistochemistry: 1:20-1:200 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:50ul
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
Specificity:Recognizes human NDUFB5. Species Crossreactivity: mouse
Isotype:IgG
Calc Applications Abbrev:E IF IHC WB
Calc Crossreactivity:Hu Mo
Immunogen:Recombinant protein corresponding to aa100-189 of human NDUFB5.