84-4864-08　H2AFY (Core Histone Macro-H2A.1, Core Histone Macro-H2A.1,Histone Macro H2A1, mH2A1, Histone H2A.y, H2A/y, Medulloblastoma Antigen MU-MB-50.205, H2AFY, MACROH2A1) 50ul　406535

特徴

• Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription.
• Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template.
• Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.
• DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
• Involved in stable X chromosome inactivation.
• Inhibits the binding of transcription factors and interferes with the activity of remodeling SWI/SNF complexes.
• Inhibits histone acetylation by EP300 and recruits class I HDACs, which induces a hypoacetylated state of chromatin.
• In addition, isoform 1, but not isoform 2, binds ADP-ribose and O-acetyl-ADP-ribose, and may be involved in ADP-ribose-mediated chromatin modulation.
• Applications:Suitable for use in ELISA, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Immunohistochemistry: 1:20-1:200 Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.