Possesses DNA-dependent ATPase and helicase activities.
This helicase translocates on single-stranded DNA in the 5￥' to 3￥' direction in the presence of ATP and, to a lesser extent, dATP.
Its unwinding activity requires a 5￥'-single-stranded region for helicase loading, since flush-ended duplex structures do not support unwinding.
The helicase activity is capable of displacing duplex regions up to 100 bp, which can be extended to 500 bp by RPA or the cohesion establishment factor, the Ctf18-RFC (replication factor C) complex activities.
Stimulates the flap endonuclease activity of FEN1.
Required for normal sister chromatid cohesion.
Required for recruitment of bovine papillomavirus type 1 regulatory protein E2 to mitotic chrmosomes and for viral genome maintenance.
Required for maintaining the chromosome segregation and is essential for embryonic development and the prevention of aneuploidy.
May function during either S, G2, or M phase of the cell cycle.
Binds to both single-and double-stranded DNA.
Applications:Suitable for use in ELISA, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry: 1:20-1:200 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, cenrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human DDX11.
Isotype：IgG
Calc Applications Abbrev：E IHC
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to aa1-220 of human DDX11.
