Cables2 (Cdk5 and ABL1 enzyme substrate 2), also known as Ik3-2, is a 478aa ubiquitously expressed protein that has a C-terminal cyclin box-containing domain, making it a member of the cyclin protein superfamily.
A related protein, Cables, forms a trimolecular complex with Cdk5 and c-Abl in vivo, enhances apoptosis induced by overexpression of p53 and may be a tumor suppressor, due to its chromosomal loss of heterozygosity that is found in certain cancers.
Cables2 shares 78% sequence similarity with Cables in the region of the cyclin box-containing domain and, in similar functionality, associates with Cdk3, Cdk5 and c-Abl.
However, unlike its close relative, Cables2 also contains an N-terminal region that enhances not only p53-mediated cell death, but also p53-independent cell death.
This characteristic suggests that the gene encoding Cables2 may also function as a tumor suppressor gene.
Applications:Suitable for use in Western Blot, Immunofluorescence, ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000 Immunofluorescence: 1:100-1:500 ELISA: 1:10,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human Ik3-2. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：E IF WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to human Ik3-2.
