84-4736-10　VAV3 (Vav 3 Oncogene, Oncogene VAV23 Guanine Nucleotide Exchange Factor, Gef, GDP/GTP Exchange Factor, Gef) 50ul　405498

特徴

• Vav proteins belong to the guanidine nucleotide exchange factor family of proteins that couple receptors to Rho-GTPases.
• The VAV family has currently three members in mammalian cells (VAV 1, VAV 2, and VAV 3).
• VAV 1 contains multiple protein binding motifs, indicating a role for VAV 1 as an adaptor protein linking cell surface receptors to downstream signaling proteins.
• VAV 1 is exclusively expressed in hematopoietic cells and plays a significant role in lymphocyte development and antigen receptor mediated activation of NFAT and NF(kappa)B, and in more delayed responses such as the production of cytokines, including IL-2, IL-3, IFN lambda, and TNF alpha.
• VAV 3 is also involved in activating JNK, p21-activated kinase (PAK) and PLC lambda, implicating a critical role in cytoskeletal rearrangement.
• VAV 3 is inducibly tyrosine phosphorylated in response to stimulation of the epidermal growth factor (EGFR), platelet-derived growth factor (PDGFR) and T-cell receptors (TCR), enhancing its guanine nucleotide exchange factor activity in vitro.
• Tyrosine 173 is the regulatory phosphorylation site of VAV 3.
• Applications:Suitable for use in Western Blot and ELISA.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1:500-1:1000ELISA: 1:10,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.