SREBP (Sterol Regulatory Element Binding Protein) 1 and 2 are transcription factors which participate in the control of cholesterol homeostasis.
SREBP proteins, which are attached to the endoplasmic reticulum and nuclear envelope, are proteolytically cleaved and thus activated in response to conditions of low cellular sterol.
SREBPs may also play some role in the apoptotic processes.
SREBP1 is an ~122kD integral membrane protein that moves from ER to golgi for processing.
The first cleavage is performed by S2P at residue 490, and the resulting active portion is then translocated out of the golgi.
This is the ~65kD mature form that is detected by this antibody.
The second cleavage is performed by S1P at residue 530, and the resulting C-term portion (from residues 530-1147) remains in the golgi where it is detected as a ~65kD protein
Applications:Suitable for use in Western Blot, Immunohistochemistry and ELISA.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:500-1:1000 Immunohistochemistry (paraffin): 1:50-1:100ELISA: 1:20,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human SREBP-1. Species Crossreactivity: rat
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Rt
Immunogen：Synthetic peptide corresponding to aa405-454 from human SREBP-1.
