AS160 is a 160kD Akt substrate that plays a critical role in regulating insulin-stimulated exocytosis of glucose transporter GLUT4.
AS160 contains a Rab GTPase-activating protein domain (GAP), suggesting its potential role in membrane trafficking.
Insulin-stimulated phosphorylation of AS160 on threonine 642 is critical for mediating GLUT4 translocation in fat and muscle cells, via inactivation of Rab GAP function.
Phosphorylation of threonine 642 is also important in mediating AS160 redistribution from low density microsomes to the cytosol in adipocytes.
Applications:Suitable for use in Immunofluorescence and ELISA.
Other applications not tested.
Recommended Dilutions:Immunofluorescence: 1:100-1:500ELISA: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human AS160. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：E IF
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to human AS160.
