Mcl-1 is an anti-apoptotic member of the Bcl-2 family of proteins.
It has a pro-survival role in the early stages of apoptosis, and is thought to prevent cytochrome c release from mitochondria.
Mcl-1 is also essential for embryogenesis and for the development and maintenance of B and T lymphocytes.
Mcl-1 is a highly regulated protein induced by a wide range of survival signals, including cytokines and growth factors.
During apoptosis, Mcl-1 is rapidly degraded by the proteasome pathway, and is also readily cleaved by caspases to produce a potent cell death promoting protein.
Applications:Suitable for use in Immunofluorescence and ELISA.
Other applications not tested.
Recommended Dilutions:Immunofluorescence: 1:100-1:500 ELISA: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels human MCL1 only when phosphorylated at Ser159. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：E IF
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to aa125-174 from human MCL1 around the phosphorylation site of Ser159.
