p90 Rsk1, a member of the RAS/MAPK signal transduction pathway, is regulated by serine/threonine phosphorylation by ERK1 and 2 in response to mitogenic stimulation.
Once phosphorylated, a significant amount of Rsk is found in the nucleus suggesting a role in nuclear signaling.
Applications:Suitable for use in Western Blot, Immunohistochemistry and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000 Immunohistochemistry: 1:50-1:100ELISA: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by peptide affinity chromatography.
Form：Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human p90 RSK only when phosphorylated at Thr359 and Ser363. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to human p90 RSK around the phosphorylation site of Thr359 and Ser363.
