The Cytomegalovirus gH pentamer complex is expressed on all wildtype hCMV strains but is notably missing from laboratory strains such as AD169 (Freed et al, 2013). This has placed severe limitations on vaccine development.
Receptor-mediated viral entry into endothelial cells requires a functional gH pentameric complex, and this complex is one of the primary targets for antiviral antibodies in infected individuals.
The gH complex comprises the gH, gL, UL128, UL130 and UL131 proteins.
Applications:Suitable for use in ELISA and Western Blot.
Other applicationsnot tested.
Recommended Dilution:ELISA: Direct or Sandwich as detection or capture antibodyOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ug
Host：sheep
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in DPBS, pH 7.4, 0.09% sodium azide.
Specificity：Recognizes Cytomegalovirus pentamer gH, gL, UL128, UL130, and UL131A proteins.
Isotype：IgG
Calc Applications Abbrev：E WB
Immunogen：Recombinant protein corresponding to Cytomegalovirus pentamer, containing gH, gL, UL128, UL130, and UL131A, expressed in mammalian cells
