These VLPs are comprised of E1 and E2 envelope proteins, along with Capsid protein.
The antibody has been shown to react with each of the components of the VLP in Western Blot analysis, when used at a 1:10,000 dilution.
Chikungunya virus is a member of the genus Alphavirus in the family Togaviridae.
Chikungunya fever is a mosquito-borne disease first identified in Tanzania in 1953.
Since 2004 there have been extensive outbreaks in Africa and Asia, and in 2013 the first cases were identified in the Caribbean, and by September 2014 more than 650,000 cases had been reported in the Americas.
Chikungunya fever usually starts 2–4 days after chikungunya virus infection, with clinical symptoms including high fever, rash, headache, vomiting, myalgia, and severe joint pain.
Because the virus is transmitted by the same mosquitos that transmit Dengue Virus and Zika Virus, and the clinical symptoms of infection with the viruses are also similar, it is important to be able to distinguish diagnostically between the different infections.
Because the use of aspirin and other NSAIDs can increase the risk of haemmorhage in Dengue fever, patients should be treated as having Dengue fever until a diagnosis of Dengue fever has been excluded.
Applications:Suitable for use in ELISA (Sandwich/Direct), Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:10,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:500ug
Host:sheep
Grade:Affinity Purified
Purity:Purified by Protein G affinity chromatography from serum.
Form:Supplied as a liquid in PBS, pH 7.4. No preservative added.
Specificity:Recognizes Chikungunya virus Capsid, E1 and E2 proteins.
Isotype:IgG
Calc Applications Abbrev:E WB
Immunogen:Recombinant protein corresponding to Chikungunya virus-like particles, containing Capsid, E1 and E2 proteins, expressed in mammalian cells. Strain Senegal 37997