84-4690-58　POLR2A (DNA-Directed RNA Polymerase II Subunit RPB1, RNA Polymerase II Subunit B1, DNA-Directed RNA Polymerase II Subunit A, DNA-Directed RNA Polymerase III Largest Subunit, RNA-Directed RNA Polymerase II Subunit RPB1, POLR2) 100ug　397521

特徴

• DNA-directed RNA polymerase II subunit RPB1, also known as RPB1, is an enzyme that in humans is encoded by the POLR2A gene.
• This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes.
• The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity.
• These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase.
• In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.
• Applications:Suitable for use in ELISA, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 0.1-0.5ug/mlImmunohistochemistry (paraffin): 0.5-1ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months after receipt at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.