Matrix metalloproteinase-2 (MMP2) is a Type IV collagenase, 72kD, which is also known as gelatinase and is a member of a group of secreted zinc metalloproteases.
The MMP2 gene is 17kb long with 13 exons varying in size from 110-901bp and 12 introns ranging from 175 to 4,350 bp, located within a region of human chromosome 16q13.
In addition, the extra exons encode the amino acids of the fibronectin-like domain which has so far been found in only the 72, 92kD type IV collagenase.
MMP2, which has a critical role in the binding of progelatinase A and TIMP4 via the C-terminal hemopexin-like domain (C domain), is functionally associated on the surface of angiogenic blood vessels.
Not only is a likely effector of endometrial menstrual breakdown, MMP2 is also effector and regulator of the inflammatory response.
Moreover, MMP2 could be helpful in diagnosing Takayasu arteritis.
Applications:Suitable for use in ELISA, Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 0.1-0.5ug/mlWestern Blot: 0.1-0.5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in 5mg/ml BSA, 0.9mg sodium chloride, 0.2mg Na2HPO4, 0.05% sodium azide. Reconstitute with 200ul sterile ddH2O to ~0.5mg/ml.
Specificity：Recognizes human MMP2.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human MMP2, (Position: K429-C660), expressed in E. coli. Species sequence homology: mouse (95.7%), rat (95.3%)
