CD2-associated protein is a protein that in humans is encoded by the CD2AP gene.
This gene encodes a scaffolding molecule that regulates the actin cytoskeleton.
The protein directly interacts with filamentous actin and a variety of cell membrane proteins through multiple actin binding sites, SH3 domains, and a proline-rich region containing binding sites for SH3 domains.
The cytoplasmic protein localizes to membrane ruffles, lipid rafts, and the leading edges of cells.
It is implicated in dynamic actin remodeling and membrane trafficking that occurs during receptor endocytosis and cytokinesis.
Haploinsufficiency of this gene is implicated in susceptibility to glomerular disease.
Applications:Suitable for use in ELISA, Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 0.1-0.5ug/mlWestern Blot: 0.1-0.5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in 4mg trehalose, 0.9mg sodium chloride, 0.2mg Na2HPO4, 0.05% sodium azide. Reconstitute with 200ul sterile ddH2O to ~0.5mg/ml.
Specificity：Recognizes human CD2AP. Species Crossreactivity: mouse, rat.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Ma Rt
Immunogen：Recombinant protein corresponding to human CD2AP, (Position: K253-K337), expressed in E. coli.
