Chromobox protein homolog 3 is a protein that is encoded by the CBX3 gene in humans.
At the nuclear envelope, the nuclear lamina and heterochromatin are adjacent to the inner nuclear membrane.
The protein encoded by this gene binds DNA and is a component of heterochromatin.
This protein also can bind lamin B receptor, an integral membrane protein found in the inner nuclear membrane.
The dual binding functions of the encoded protein may explain the association of heterochromatin with the inner nuclear membrane.
This protein binds histone H3 tails methylated at Lys-9 sites.
This protein is also recruited to sites of ultraviolet-induced DNA damage and double-strand breaks.
Two transcript variants encoding the same protein but differing in the 5' UTR, have been found for this gene.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 0.1-0.5ug/mlWestern Blot: 0.1-0.5ug/mlImmunohistochemistry (paraffin): 0.5-1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in 4mg trehalose, 0.9mg sodium chloride, 0.2mg Na2HPO4, 0.05% sodium azide. Reconstitute with 200ul sterile ddH2O to ~0.5mg/ml.
Specificity：Recognizes human CBX3. Species Crossreactivity: mouse, rat.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to human CBX3, (Position: A2-Q183), expressed in E. coli.
