84-4688-80　BEST1 (Bestrophin-1, TU15B, Vitelliform Macular Dystrophy Protein 2, VMD2) 100ug　397336

特徴

• Bestrophin-1 (Best1) is a protein that, in humans, is encoded by the BEST1 gene.
• This gene encodes a member of the bestrophin gene family.
• This small gene family is characterized by proteins with a highly conserved N-terminal with four to six transmembrane domains.
• Bestrophins may form chloride ion channels or may regulate voltage-gated L-type calcium-ion channels.
• Bestrophins are generally believed to form calcium-activated chloride-ion channels in epithelial cells but they have also been shown to be highly permeable to bicarbonate ion transport in retinal tissue.
• Mutations in this gene are responsible for juvenile-onset vitelliform macular dystrophy (VMD2), also known as Best macular dystrophy, in addition to adult-onset vitelliform macular dystrophy (AVMD) and other retinopathies.
• Alternative splicing results in multiple variants encoding distinct isoforms.
• Applications:Suitable for use in Flow Cytometry, Western Blot, Immunohistochemistry, Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:Flow Cytometry: 1-3ug/1x106 cells Western Blot: 0.1-0.5ug/mlImmunohistochemistry (frozen): 0.5-1ug/mlImmunocytochemistry: 0.5-1ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months after receipt at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.