Angiogenin (Ang), also known as ribonuclease 5, is a small 123aa protein that in humans is encoded by the ANG gene.
The protein encoded by this gene is an exceedingly potent mediator of new blood vessel formation.
It hydrolyzes cellular tRNAs resulting in decreased protein synthesis and is similar to pancreatic ribonuclease.
In addition, the mature peptide has antimicrobial activity against some bacteria and fungi, including S.
pneumoniae and C.
albicans.
Alternative splicing results in two transcript variants encoding the same protein.
This gene and the gene that encodes ribonuclease, RNase A family, 4 share promoters and 5' exons.
Each gene splices to a unique downstream exon that contains its complete coding region.
Applications:Suitable for use in ELISA, Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 0.1-0.5ug/mlWestern Blot: 0.1-0.5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in 5mg/ml BSA, 0.9mg sodium chloride, 0.2mg Na2HPO4, 0.05% sodium azide. Reconstitute with 200ul sterile ddH2O to ~0.5mg/ml.
Specificity：Recognizes human ANG.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human ANG, (Position: Q25-P147), expressed in E. coli. Species sequence homology: mouse (77.5%).
