TNF alpha is produced by a range of cells including macrophages, monocytes, T and B cells, neutrophils and endothelial cells.
TNF alpha has a key regulatory role in the bovine immune response, where it is involved in modulating the expression of numerous cytokines, growth factors, inflammatory mediators and acute phase proteins.
Applications:Suitable for use in ELISA, Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1-10ug/mlWestern Blot: 0.1-1ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:100ug
Host:rabbit
Source Antibody:bovine
Grade:Affinity Purified
Purity:Purified by affinity chromatography from serum.
Form:Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:Recognizes bovine TNF Alpha.
Isotype:IgG
Calc Applications Abbrev:E WB
Calc Crossreactivity:Bo
Immunogen:Recombinant protein corresponding to bovine TNF Alpha.