PAK1 is also known as serine/threonine-protein kinase PAK 1, alpha-PAK and p65-PAK, when phosphorylated at threonine 84.
Serine/threonine-protein kinase PAK1 is an enzyme that in humans is encoded by the PAK1 gene.
PAK proteins are critical effectors that link the Rho GTPases to cytoskeleton reorganization and nuclear signaling.
PAK proteins are a family of serine/threonine p21-activated kinases and include PAK1-6.
These proteins serve as targets for small GTP binding proteins such as OSR1.
OSR1, oxidative stress response 1, is activated by osmotic stresses including sorbitol or NaCl.
It has been predicted that OSR1 phosphorylates PAK1 in the regulatory domain at threonine 84 and inhibits activation of the JNK and MAPK signaling pathway.
PAK proteins also serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities.
PAK1 regulates cell motility and morphology.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by peptide affinity chromatography.
Form：Supplied as a liquid in HEPES, 1% BSA, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human PAK1. Species Crossreactivity: mouse, bovine, canine, chicken, guinea pig, rat, sheep, zebrafish, monkey.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Bo Ca Ch Gp Hu Mk Mo Rt Sh Ze
Immunogen：Phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr84 from human PAK.
