p62 is important in regulating critical cellular functions, including bone homeostasis, obesity, and cancer via its interactions with various signaling intermediaries.
The SQSTM1 gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappaB (NFkappaB) signaling pathway.
p62 functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NFkappaB in response to upstream signals.
p62 plays an important role in bone remodeling, regulating this process through its role in a chemical signaling pathway that promotes the formation of osteoclasts.
p62 may be involved autophagy, the self-destruction of cells (apoptosis), and the body's immune responses and inflammatory reactions.
p62 is an integral component of inclusions in brains of various neurodegenerative disorders, including Alzheimer disease (AD) neurofibrillary tangles (NFTs) and Lewy bodies in Parkinson disease.
p62 plays an important role in the protection of cells from the toxicity of misfolded proteins by enhancing aggregate formation especially in the later stages.
p62 is overexpressed in many human cancers and is induced during cell transformation.
cdk1 phosphorylates p62 in vitro and in vivo at T269 and S272, which is necessary for the maintenance of appropriate cyclin B1 levels and the levels of cdk1 activity necessary to allow cells to properly enter and exit mitosis.
The lack of cdk1-mediated phosphorylation of p62 leads to a faster exit from mitosis, translating into enhanced cell proliferation and tumorigenesis in response to Ras-induced transformation.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by peptide affinity chromatography.
Form：Supplied as a liquid in HEPES, 1% BSA, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes ubiquitin-binding protein p62, also known as sequestosome-1 and EBI3-associated protein of 60kD (EBIAP), when phosphorylated at threonine 269 and serine 272.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr269/Ser272 from human p62. Species sequence homology: primate.
