Cdc2, a serine/threonine kinase, plays a crucial role in regulation of the eukaryotic cell cycle by phosphorylating proteins involved in DNA replication and other important cell cycle progression processes.
Binding of cyclins to Cdc2 is essential for its regulation, and the kinase only becomes activated once bound to cyclins.
Cyclin binding has also been reported to play a role in substrate recruitment.
Cdc2 activity is highly regulated through phosphorylation by the serine/threonine protein kinase Wee1.
This phosphorylation results in Cdc2 inactivation and consequently, cell cycle arrest at the G2/M transition stage.
Wee1 phosphorylation is counteracted by the Cdc25 phosphatase, which dephosphorylates Cdc2 thereby enabling entry into mitosis.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:400Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.2% gelatin, 0.09% sodium azide.
Specificity：Recognizes human Cdc2. Species Crossreactivity: mouse, rat.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to human Cdc2, at C-terminal.
