The c-Fos transcription factor is encoded by the c-Fos proto-oncogene and dimerizes with c-Jun, JDP and ATF family members to form the AP-1 transcription factor.
c-Fos plays an essential role in mediating cellular signaling responses after cellular stress.
The ability of the c-Fos protein to act as a transcription factor is regulated by phosphorylation on selected sites by the kinases p38, ERK, RSK1 and RSK2.
The protein accumulates in the nucleus, where it acts as a transcription factor upon formation of heterodimers with c-Jun.
This binding prevents the nuclear export and subsequent proteosomal degradation of c-Fos.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.2% gelatin, 0.09% sodium azide.
Specificity：Recognizes human C-Fos. Species Crossreactivity: mouse, rat.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to human c-Fos, at N-terminal.
