The ATF2 gene encodes a transcription factor that is a member of the leucine zipper family of DNA-binding proteins.
This protein binds to the cAMP-responsive element (CRE), an octameric palindrome.
The protein forms a homodimer or heterodimer with c-Jun and stimulates CRE-dependent transcription.
Several studies implicate abnormal activation of ATF-2 in growth and progression of mammalian skin tumors.
ATF2 may mediate oncogenesis caused by mutant Ras protein and regulate maintenance of the aggressive cancer phenotype of some types of epithelial cells.
ATF2 has also been shown to be phosphorylated by ATM at Serine 490 and 498 in humans.
The serine phosphorylated forms play a role in mediating DNA repair and in response to DNA damage induced by ionizing radiation (IR).
ATF2 exhibits histone acetyltransferase (HAT) activity and has been reported to specifically acetylate histones H2B and H4 in vitro.
In concert with CUL3 and RBX1, ATF2 promotes the degradation of KAT5 thereby attenuating its ability to acetylate and activate ATM.
This protein can also elicit oncogenic or tumor suppressor activities depending on the tissue or cell type.
In response to stress stimuli, ATF2 activates a variety of gene targets that are involved in oncogenesis, and has been correlated with maintenance of a cancer cell phenotype.
Inhibiting ATF2 impedes melanoma development and elicits tumor suppressorfunction.
To act as a tumor suppressor, ATF2 must localize at the mitochondria and phosphorylation at Thr52 by PKCepsilon regulates this translocation.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:250Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by peptide affinity chromatography.
Form：Supplied as a liquid in HEPES, 1% BSA, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human ATF2, when phosphorylated at threonine 52. Species sequence homology: bovine, chicken, mouse, monkey, rat, xenopus.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Bo Ch Hu Mk Mo Rt Xe
Immunogen：Phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr269/Ser272 from human ATF2.
