The protein encoded by this gene binds proteins of the TEA domain family of transcription factors (TEFs) through the Vg (vestigial) homology region found in its N-terminus.
It may thus function as a specific coactivator for the mammalian TEFs.
TDU interacted directly with the TEA domain family member, TEF1, and deletion of the Vg homology region abolished the interaction.
The TDU-TEF1 dimer activated a reporter plasmid, and expression of TDU in Drosophila rescued loss of Vg function.
The interaction was stronger in cardiac myocytes, suggesting a myocyte-specific factor may participate in the interaction.
Vgll1 was weakly active in driving expression of a reporter gene from the mouse skeletal muscle alpha-actin promoter, and Vgll1 could partially reverse the inhibitory effect of TEF1 in this assay.
Applications:Suitable for use in ELISA, Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:2000-1:10,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：20ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.05% sodium azide, 50% glycerol.
Specificity：Recognizes human VGLL1.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to human VGLL1.
