Spata17 gene, which called MSRG11, by digital differential display of ESTs found only in testis.
The deduced 292-amino acid protein was considered to be a member of the family of calmodulin (CaM)-binding proteins because it contained 3 short CaM-binding motifs containing conserved ile and gln residues (IQ motif).
Spata17 protein was most abundant in the cytoplasm of round spermatids and elongating spermatids within seminiferous tubules of the adult testis.
Expression gradually decreased in the manipulated testis of animals that underwent experimental unilateral cryptorchidism.
Transient transfection experiments with a Spata17 expression construct in cultured spermatogonia GC-1 cells indicated that Spata17 accelerated apoptosis in a dose-dependent manner.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:200-1:1000Immunohistochemistry: 1:10-1:50Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.05% sodium azide, 50% glycerol.
Specificity：Recognizes human SPATA17.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human SPATA17.
