This gene encodes a testis-specific protein that belongs to the tetratricopeptide repeat-like superfamily.
The encoded protein localizes to the Golgi apparatus and may play a role in spermatogenesis.
The SPATA16 protein contains a region of homology to the mouse male enhanced antigen-2, a SCOP domain belonging to the tetratricopeptide repeat-like superfamily, 3 glycosylation sites, and several phosphorylation sites.
The mouse and human proteins share 77% sequence identity.
Northern blot analysis of multiple human tissues detected a strong signal in testis and weak expression in pancreas and kidney.
Laser confocal microscopy localized a GFP-SPATA16 fusion protein specifically to the Golgi apparatus.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:200-1:1000Immunohistochemistry: 1:15-1:50Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:20ul
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.3, 0.05% sodium azide, 50% glycerol.
Specificity:Recognizes human SPATA16. Species Crossreactivity: mouse and rat
Isotype:IgG
Calc Applications Abbrev:E IHC WB
Calc Crossreactivity:Hu Mo Rt
Immunogen:Recombinant protein corresponding to human SPATA16.