84-4639-01　［取扱停止］RNF144B 200ul　393929

特徴

• p53 is the most commonly mutated gene in human cancer identified to date.
• Expression of p53 leads to inhibition of cell growth by preventing progression of cells from G1 to S phase of the cell cycle.
• Most importantly, p53 functions to cause arrest of cells in the G1 phase of the cell cycle following any exposure of cells to DNA-damaging agents.
• The MDM2 (murine double minute-2) protein was initially identified as an oncogene in a murine transformation system.
• MDM2 functions to bind p53 and block p53-mediated transactivation of cotransfected reporter constructs.
• The MDM2 gene is amplified in a high percentage of human sarcomas that retain wildtype p53 and tumor cells that overexpress MDM2 can tolerate high levels of p53 expression.
• Another p53 target protein is the p53-inducible RING finger protein (p53RFP), an auto-ubiquitinylated protein acting as an E3 ubiquitin ligase.
• p53RFP, also designated IBRDC2 in mouse and rat, receives ubiquitin from specific E2 ubiquitin-conjugating enzymes and transfers it to substrates that promote their degradation by the proteasome.
• p53RFP may mediate re-entry into the cell cycle.
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:200-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.