84-4627-28　［取扱停止］PRKAR1A 20ul　393349

特徴

• The second messenger cyclic AMP (cAMP) activates cAMP-dependent protein kinase (PKA or cAPK) in mammalian cells and controls many cellular mechanisms such as gene transcription, ion transport, and protein phosphorylation.
• Inactive PKA is a heterotetramer composed of a regulatory subunit (R) dimer and a catalytic subunit (C) dimer.
• In this inactive state, the pseudosubstrate sequences on the R subunits block the active sites on the C subunits.
• Three C subunit isoforms (C-alpha, C-beta, and C-gamma) and two families of regulatory subunits (RI and RII) with distinct cAMP binding properties have been identified.
• The two R families exist in two isoforms, alpha and beta (RI-alpha, RI-beta, RII-alpha, and RII-beta).
• Upon binding of cAMP to the R subunits, the autoinhibitory contact is eased and active monomeric C subunits are released.
• PKA shares substrate specificity with Akt (PKB) and PKC, which are characterized by an arginine at position -3 relative to the phosphorylated serine or threonine residue.
• Substrates that present this consensus sequence and have been shown to be phosphorylated by PKA are Bad (Ser155), CREB (Ser133), and GSK-3 (GSK-3alpha Ser21 and GSK-3beta Ser9).
• In addition, combined knock-down of PKA C-alpha and -beta blocks cAMP-mediated phosphorylation of Raf (Ser43 and Ser259).
• Autophosphorylation and phosphorylation by PDK-1 are two known mechanisms responsible for phosphorylation of the C subunit at Thr197.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.