The microtubule matrix within a cell plays a central role in intracellular transport, cell shape during differentiation and chromosome partitioning during mitosis.
During these processes, microtubules transition rapidly between stable and dynamic states.
MAP/microtubule affinity-regulating kinase 1 (MARK1) is a 795 amino acid protein belonging to the CAMK Ser/Thr protein kinase family.
MARK1 is thought to play a role in the stability of the microtubule matrix of the cytoskeleton.
MARK1 is activated by phosphorylation of Thr215 by LKB1 in complex with STRAD and MO25.
Localized to the cytoskeleton, MARK1 contains one kinase-associated (KA1) domain, one protein kinase domain and one UBA domain.
Expressed as three isoforms produced by alternative splicing, MARK1 is found with highest levels in brain, skeletal muscle and heart.
Applications:Suitable for use in ELISA, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.05% sodium azide, 50% glycerol.
Specificity：Recognizes human MARK1.
Isotype：IgG
Calc Applications Abbrev：E IHC
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to human MARK1.
