84-4580-67　［取扱停止］LCK 20ul　391042

特徴

• Non-receptor tyrosine-protein kinase that plays an essential role in the selection and maturation of developing T-cells in the thymus and in the function of mature T-cells.
• Plays a key role in T-cell antigen receptor (TCR)-linked signal transduction pathways.
• Constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors.
• Association of the TCR with a peptide antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, thereby recruiting the associated LCK protein to the vicinity of the TCR/CD3 complex.
• LCK then phosphorylates tyrosines residues within the immunoreceptor tyrosine-based activation motifs (ITAM) of the cytoplasmic tails of the TCR-gamma chains and CD3 subunits, initiating the TCR/CD3 signaling pathway.
• Once stimulated, the TCR recruits the tyrosine kinase ZAP70, that becomes phosphorylated and activated by LCK.
• Following this, a large number of signaling molecules are recruited, ultimately leading to lymphokine production.
• LCK also contributes to signaling by other receptor molecules.
• Associates directly with the cytoplasmic tail of CD2, which leads to hyperphosphorylation and activation of LCK.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.