84-4572-41　［取扱停止］ISG15 20ul　390633

特徴

• Interferon-stimulated 15kD protein (ISG15), also known as ubiquitin cross-reactive protein (UCRP), is a member of the ubiquitin-like protein family and functions in various biological pathways from pregnancy to innate immune responses.
• Expression of ISG15 is stimulated by cellular exposure to type 1 interferons alpha and beta, in addition to infection with viruses such as influenza B.
• After exposure to type I interferons, both lymphocytes and monocytes, in addition to some fibroblasts and epithelial cells, release ISG15 into culture medium.
• ISG15 has been shown to function as a cytokine, stimulating interferon gamma secretion by monocytes and macrophages, proliferation of natural killer cells, and chemotactic responses in neutrophils).
• ISG15 has also been shown to function intracellularly, being covalently conjugated to other proteins by E1 (Ube1L), E2 (UbcH8) and E3 ligases via a multi-step process analogous to ubiquitination).
• ISG15 is removed from proteins by the ubiquitin processing protease Ubp43.
• ISG15-protein conjugation (ISGylation) is induced by type 1 interferons, and target proteins include the serine protease inhibitor Serpin 2A, PLCgamma1, ERK1/2, Jak1 and Stat1.
• Unlike ubiquitination, ISGylation does not target proteins for degradation, rather ISGylation increases Jak1 and Stat1 activity, enhancing the cellular response to interferons.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.