84-4566-57　［取扱停止］IGSF6 20ul　390344

特徴

• IGSF6 (DORA), a novel member of the immunoglobulin superfamily (IGSF) from human and rat expressed in dendritic and myeloid cells.
• Using a probe from the open reading frame of the rat cDNA, we isolated a cosmid which contains the entire mouse gene.
• By comparative analysis and reverse transcriptase polymerase chain reaction, we defined the intron/exon structure and the mRNA of the mouse gene and, with respect to human BAC clones, the human gene.
• The genes span 10 kb (mouse) and 12 kb (human), with six exons arranged in a manner similar to other members of the IGSF.
• All intron/exon boundaries follow the GT-AG rule.
• Expression of the mouse Igsf6 gene is restricted to cells of the immune system, particularly macrophages.
• Northern blot revealed a single mRNA of 2.5 kb, in contrast to the human gene which is expressed as two mRNAs of 1 and 2.5 kb.
• The human and mouse genes were localized to a locus associated with inflammatory bowel disease.
• Analysis of the flanking regions of the Igsf6 gene revealed the presence of an unrelated gene, transcribed from the opposite strand of the DNA and oriented such that the Igsf6 gene is encoded entirely within an intron.
• An identical organization is seen in human.
• Applications:Suitable for use in ELISA, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Immunohistochemistry: 1:5-1:20Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.